Tuesday, October 19, 2004
Let's talk Xylenol Orange
As I have a lot of articles to review and submit in the next months, I decided to take and advanced written communication course which brings me HERE.
For this blog, I will attempt to note the progress of my research and writtting on a methodology article for the quantification of hydroperoxides of fatty acids.
For this blog, I will attempt to note the progress of my research and writtting on a methodology article for the quantification of hydroperoxides of fatty acids.
Yin and Porter, 2003
From
Yin, H. and Porter, N.A. (2003) Specificity of the ferrous oxidation of xylenol orange assay: analysis of autoxidation products of cholesteryl arachidonate. Analytical Biochemistry 313, 319-326.
Authors
Both from the Department of chemistry and Center in Molecular Toxicology, Nashville
Audience
This article must have been written for analytical chemists and biochemists that are in need of quantifying hydroperoxides, most probably of lipids.
Topic
To determine if the FOX or the TBARS are specific methods for the quantification of hydroperoxides. They found that cyclic peroxides give a positive response using the FOX method, even with the use of triphenylphosphine. Thus that the FOX assay was not specific for the quantification of hydroperoxides.
Purpose
To provide support as to the caution that must be taken when using the FOX method, specially for highly oxidized lipids, such as theones that contain arachidonyl groups.
Rationale
False positive response using methods for quantification of the hydroperoxides must be assessed due to the fact that quantification of hydroperoxides in plasma, for instance, cannot be controlled by a blank. As they mention in their text: "Mounting evidence shows that lipid peroxidation products diretly and indirectly affect many functions..."
Comment/Input
This text helps to contextualise the experimental work that could be designed in order to find the appropriate method of quantification of the activity of LOX. As the activity of the enzyme can be measured and controlled by a blank, the idea now is to make sure we have the appropriate blank.
Yin, H. and Porter, N.A. (2003) Specificity of the ferrous oxidation of xylenol orange assay: analysis of autoxidation products of cholesteryl arachidonate. Analytical Biochemistry 313, 319-326.
Authors
Both from the Department of chemistry and Center in Molecular Toxicology, Nashville
Audience
This article must have been written for analytical chemists and biochemists that are in need of quantifying hydroperoxides, most probably of lipids.
Topic
To determine if the FOX or the TBARS are specific methods for the quantification of hydroperoxides. They found that cyclic peroxides give a positive response using the FOX method, even with the use of triphenylphosphine. Thus that the FOX assay was not specific for the quantification of hydroperoxides.
Purpose
To provide support as to the caution that must be taken when using the FOX method, specially for highly oxidized lipids, such as theones that contain arachidonyl groups.
Rationale
False positive response using methods for quantification of the hydroperoxides must be assessed due to the fact that quantification of hydroperoxides in plasma, for instance, cannot be controlled by a blank. As they mention in their text: "Mounting evidence shows that lipid peroxidation products diretly and indirectly affect many functions..."
Comment/Input
This text helps to contextualise the experimental work that could be designed in order to find the appropriate method of quantification of the activity of LOX. As the activity of the enzyme can be measured and controlled by a blank, the idea now is to make sure we have the appropriate blank.
